The robot asks the children if any of them wondered which of The Three Stooges had the heaviest brain. Nelson imagines himself as a scientist trying to answer the question and slaps the brains, parodying the slapstick style of humor from The Three Stooges.
A slide shaped like the double helix can be seen in the museum.
The ceiling of the museum was donated by Velcro, while the floor is made with Super Ball material.
The arcade game in the Sex Education Pavilion looks similar to the AG-2G quad laser cannon on Han Solo's ship, the Millennium Falcon, in the Star Wars series.
The "Mars" sign uses the same font as in the Star Wars logo.
Homer tries one of the messages in 99 Hilarious Phone Messages which says "This is Jerry Maguire. Show me the message. Show me the message!". This is a pun on "Show me the money. Show me the money!", a famous line by Jerry MaGuire (Tom Cruise) in the 1996 sports comedy-drama film Jerry Maguire.
Marge imitates Geraldine Jones from The Flip Wilson Show. The phrase "The devil made me miss your call" is a pun on "The Devil made me buy this dress!", one of Geraldine's most famous quotes.
For the last answer message, Homer shouts "Do the message!" and starts humming Van McCoy's "The Hustle", a disco song from 1975. The original lyrics say "Do the Hustle!" right before the iconic disco tune starts.
After seeing that Ralph does not understand how Go Fish is played, Bart tries to put Super Friends on TV. This animated TV series featured the Justice League of superheroes.
When Skinner is introducing the robot to the class, Bart's desk is clearly in the middle of the room. When Bart throws the apple to the man in the tree, he's sitting next to the window.
As Troy McClure is talking and the Simpson family is on the Knowledgium runway, Maggie's bow is colored yellow.
The door leading to the electric chair initially has " Danger" written in red text, but after Bart opens the door it is written in black text.